fa بررسی تأثیر مهار گیرنده A2a مرکزی بر سندرم محرومیت از مصرف مزمن مرفین در موش صحرائی تخصصي Special پژوهشي Research <p class="MsoNormal" dir="rtl" style="text-align: right direction: rtl unicode-bidi: embed"><font face="tahoma, arial, helvetica, sans-serif" size="3"><b><span lang="FA">چکیده</span></b><b><span dir="ltr"><o:p /></span></b></font></p><p class="MsoNormal" dir="rtl" style="direction: rtl unicode-bidi: embed"><font face="tahoma, arial, helvetica, sans-serif" size="3"><b><span lang="AR-SA">زمینه و هدف:</span></b><span lang="AR-SA"> هدف</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">اصلی</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">از</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">این</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">مطالعه</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">بررسی</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">تأثیر</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">تزریق</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">داخل</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">بطن</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">مغزی </span><span dir="ltr">SCH-58261</span><span dir="rtl"></span><span dir="rtl"></span><span lang="AR-SA"><span dir="rtl"></span><span dir="rtl"></span> به عنوان آنتاگونیست گیرنده­های مرکزی آدنوزینی</span><span dir="ltr"></span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> A2a</span><span lang="AR-SA">بر سندرم محرومیت از مصرف مرفین در</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">موش صحرائی نر بود.<o:p /></span></font></p><p class="MsoNormal" dir="rtl" style="direction: rtl unicode-bidi: embed"><font face="tahoma, arial, helvetica, sans-serif" size="3"><b><span lang="AR-SA">روش</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span></b><b><span lang="AR-SA">بررسی</span></b><span dir="ltr"></span><span dir="ltr"></span><b><span dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>:</span></b><span dir="rtl"></span><span dir="rtl"></span><span lang="AR-SA"><span dir="rtl"></span><span dir="rtl"></span> رت</span><span lang="FA">های</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">نر، نژاد ویستار درمحدوده</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">وزنی </span><span dir="ltr">gr</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA"><span dir="rtl"></span><span dir="rtl"></span>250-200 </span><span lang="AR-SA">و</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">با</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">سن</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">مشابه که بصورت تصادفی انتخاب شده</span><span dir="ltr">‌</span><span lang="AR-SA">اند، به </span><span lang="FA">6</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">گروه هشت</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">تائی</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">تقسیم</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">شد</span><span lang="FA">ند. </span><span lang="AR-SA">به منظور ایجاد وابستگی، </span><span lang="FA">مرفین</span><span lang="AR-SA"> روزی دو بار به مدت 9 روز </span><span lang="FA">(روز اول: </span><span dir="ltr"></span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> mg/kg</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA"><span dir="rtl"></span><span dir="rtl"></span>5، روز دوم و سوم: </span><span dir="ltr"></span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> mg/kg</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA"><span dir="rtl"></span><span dir="rtl"></span>10، روز چهارم و پنجم</span><span dir="ltr">mg/kg :</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA"><span dir="rtl"></span><span dir="rtl"></span>15، روز ششم و هفتم: </span><span dir="ltr"></span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> mg/kg</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA"><span dir="rtl"></span><span dir="rtl"></span>20 و روز هشتم :</span><span dir="ltr"></span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> mg/kg </span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA"><span dir="rtl"></span><span dir="rtl"></span>25 و در روز نهم فقط دوز صبحگاهی مرفین </span><span dir="ltr">mg/kg</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA"><span dir="rtl"></span><span dir="rtl"></span>25</span><span lang="AR-SA">) </span><span lang="FA">تزریق شد</span><span lang="AR-SA">.</span><span lang="AR-SA"> </span><span dir="ltr">SCH-58261</span><span dir="rtl"></span><span dir="rtl"></span><span lang="AR-SA"><span dir="rtl"></span><span dir="rtl"></span> در دوزهای (</span><span dir="ltr">µl</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA"><span dir="rtl"></span><span dir="rtl"></span>5</span><span dir="ltr">µg/</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA"><span dir="rtl"></span><span dir="rtl"></span> 40 و 80 و 160</span><span lang="AR-SA">) در روز نهم</span><span lang="FA">،</span><span lang="AR-SA"> نیم ساعت بعد از آخرین دوز مرفین، تزریق</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">داخل</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">بطن</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">مغزی گردید. پس از نیم ساعت نالوکسان</span><span dir="ltr">mg/kg_, ip) </span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA"><span dir="rtl"></span><span dir="rtl"></span>4 </span><span dir="ltr"></span><span dir="ltr"></span><span dir="ltr"><span dir="ltr"></span><span dir="ltr"></span>(</span><span dir="rtl"></span><span dir="rtl"></span><span lang="FA"><span dir="rtl"></span><span dir="rtl"></span> تزریق شده و علایم قطع مصرف شامل ایستادن روی پاها، کشیدن شکم روی زمین، حرکات شبیه سگ خیس، تیمار کردن آلت تناسلی</span><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="FA">و دندان قروچه به مدت یک ساعت ثبت گردید<o:p /></span></font></p><p class="MsoNormal" dir="rtl" style="direction: rtl unicode-bidi: embed"><font face="tahoma, arial, helvetica, sans-serif" size="3"><b><span lang="FA">یافته­ ها:</span></b><span lang="FA"> نتایج مطالعه حاضر نشان داد تزریق روزانه مرفین به روش دوزهای فزاینده سبب ایجاد وابستگی و متعاقب تزریق نالوکسان بروز علایم قطع مصرف شد که با گروه دریافت کننده سالین تفاوت معنی­داری در بروز علایم و امتیاز تام سندرم ترک داشت. همچنین نتایج نشان داد که تزریق </span><span lang="AR-SA">داخل</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">بطن</span><span dir="ltr"></span><span dir="ltr"></span><span lang="AR-SA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">مغزی آنتاگونیست گیرنده های آدنوزینی بطور معنی­دار (</span><span lang="FA">0.001</span><span lang="AR-SA">></span><span dir="ltr">p</span><span dir="rtl"></span><span dir="rtl"></span><span lang="AR-SA"><span dir="rtl"></span><span dir="rtl"></span>) سبب کاهش علایم تام سندرم ترک گردید</span><span lang="FA"> <o:p /></span></font></p><p class="MsoNormal" dir="rtl" style="text-align: right direction: rtl unicode-bidi: embed"> </p><p class="MsoNormal" dir="rtl" style="direction: rtl unicode-bidi: embed"><font face="tahoma, arial, helvetica, sans-serif" size="3"><b><span lang="FA">نتیجه ­گیری:</span></b><span dir="ltr"></span><span dir="ltr"></span><span lang="FA" dir="ltr"><span dir="ltr"></span><span dir="ltr"></span> </span><span lang="AR-SA">در مجموع نتایج نشان داد که بکارگیری </span><span dir="ltr">SCH-58261</span><span dir="rtl"></span><span dir="rtl"></span><span lang="AR-SA"><span dir="rtl"></span><span dir="rtl"></span> به عنوان آنتاگونیست گیرنده </span><span dir="ltr">A_2a</span><span dir="rtl"></span><span dir="rtl"></span></font><span lang="AR-SA"><font face="tahoma, arial, helvetica, sans-serif" size="3"><span dir="rtl"></span><span dir="rtl"></span> آدنوزینی علایم قطع مصرف را کاهش داده است.</font><span style="font-family: 'B Zar' font-size: 10.5pt"><o:p /></span></span></p> <font size="3" face="times new roman, times, serif"><b> Abstract</b></font><div><font size="3" face="times new roman, times, serif"><b>Background and Aim:</b> The aim of this study was to evaluate the effect of intracerebroventricular (icv) administration of antagonist of A2a receptore on morphine withdrawal syndrome in male rat.</font></div><div><font size="3" face="times new roman, times, serif"><b>Method: </b>Male wistar rats (200-250 gr) were selected randomly and divided into six groups (n=8). Morphine was administered twice a day for nine days (day 1: 5mg/kg/12h, day 2,3: 10mg/kg/12h, day 4,5: 15 mg/kg/12h, day 6,7: 20mg/kg/12h, day 8,9: 25 mg/kg/12h). SCH-58261)40, 80, 160µg/5µl/Rat) injected interacerebroventricular on 9th day, 30 minute after the last dose of morphine. Tirtheen minutes later, naloxone (4mg/kg, ip) injected and the withdrawal signs (Rearing, Genital Grooming, Abdomen Writhing, Teeth Chattering and Wet Dog Shake) were recorded for 60 minutes. </font></div><div><font size="3" face="times new roman, times, serif"><b>Result:</b> </font><span style="font-family: 'times new roman', times, serif font-size: medium">Results showed that chronic morphine administration for nine days induced morphine dependency and naloxone injection percipitated withdrawal symptoms in dependent rats. Also the results indicated that intracerebroventricular administration of A2a receptore antagonist (SCH-58261) reduced all morphine-induced withdrawal symptoms.  </span></div><div><span style="font-family: 'times new roman', times, serif font-size: medium"><b>Conclusion:</b> In conclusion we found that central injection of SCH-58261 as an A2a receptore antagonist decreased morphine withdrawal symptoms in rats.</span></div> مرفین,SCH-58261, تزریق داخل بطن مغزی, علائم سندرم محرومیت Intracerebroventricular, morphine, SCH-58261, withdrawal symptoms 8 18 http://zanko.muk.ac.ir/browse.php?a_code=A-10-1-4&slc_lang=fa&sid=1 M Charkhpour محمد چرخ‌پور 100319475328460061 100319475328460061 No A Parvizpur علیرضا پرویزپور 100319475328460062 100319475328460062 No Z Namjo زهرا نامجو 100319475328460063 100319475328460063 No S Moradi صباح مرادی 100319475328460064 100319475328460064 No K Hassanzadeh کامبیز حسن زاده kambizhassanzadeh@gmail.com 100319475328460065 100319475328460065 Yes