Volume 20, Issue 65 (7-2019)
Zanko J Med Sci 2019, 20(65): 20-29 |
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Jafari-Sales A, jafari B, Bagherizadeh Y, Khalifehpour M, Abdoli-senejan M, Helali-Pargali R. Antibiotic resistance pattern and bla-TEM gene expression in Acinetobacter baumannii isolated from clinical specimens of Tabriz hospitals. Zanko J Med Sci 2019; 20 (65) :20-29
URL: http://zanko.muk.ac.ir/article-1-434-en.html
URL: http://zanko.muk.ac.ir/article-1-434-en.html
Abolfazl Jafari-Sales * 
, Behboud Jafari , Yashar Bagherizadeh , Masood Khalifehpour , Mahboubeh Abdoli-senejan , Raziyeh Helali-Pargali
, Behboud Jafari , Yashar Bagherizadeh , Masood Khalifehpour , Mahboubeh Abdoli-senejan , Raziyeh Helali-Pargali
Abstract: (3370 Views)
Background and Aim: Acinetobacter species are potentially problematic pathogens in multiple hospital infections. This bacterium often resists several classes of antibiotics such as beta-lactams, which is an emerging problem in Iran. Therefore, the aim of this study was to determine the pattern of resistance and prevalence of bla-TEM gene in Acinetobacter isolated from Tabriz hospitals.
Material and Method: This descriptive cross-sectional study was performed on 148 isolates of Acinetobacter isolated from different clinical specimens. Samples were isolated and identified using standard microbiology laboratory methods and culture on specific environments. Antibiotic resistance patterns of the isolated were defined by Kirby-Bauer disc diffusion method. In order to identify the bla-TEM gene, the combination method was used as a phenotypic method. Additionally, PCR method was used to identify the genotypes.
Results: Of 115 isolates (77.7%) of Acinetobacter baumannii, the highest sensitivity was for cefotaxime (100%) and ceftazidime (100%) antibiotics. The combined test results showed that 10 (8.7%) isolates were producing broad-spectrum beta-lactamases (ESBLs), and 36 (31.3%) isolates had the bla-TEM gene.
Conclusion: By increasing the prevalence of resistance to antibiotics, rapid and timely detection of antibiotic-resistant strains is necessary in order to select appropriate treatments and prevent spreading of the resistance.
Material and Method: This descriptive cross-sectional study was performed on 148 isolates of Acinetobacter isolated from different clinical specimens. Samples were isolated and identified using standard microbiology laboratory methods and culture on specific environments. Antibiotic resistance patterns of the isolated were defined by Kirby-Bauer disc diffusion method. In order to identify the bla-TEM gene, the combination method was used as a phenotypic method. Additionally, PCR method was used to identify the genotypes.
Results: Of 115 isolates (77.7%) of Acinetobacter baumannii, the highest sensitivity was for cefotaxime (100%) and ceftazidime (100%) antibiotics. The combined test results showed that 10 (8.7%) isolates were producing broad-spectrum beta-lactamases (ESBLs), and 36 (31.3%) isolates had the bla-TEM gene.
Conclusion: By increasing the prevalence of resistance to antibiotics, rapid and timely detection of antibiotic-resistant strains is necessary in order to select appropriate treatments and prevent spreading of the resistance.
Type of Study: Research |
Subject:
Special
Received: 2018/10/25 | Accepted: 2018/12/24 | ePublished: 2019/07/15
Received: 2018/10/25 | Accepted: 2018/12/24 | ePublished: 2019/07/15
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