Volume 23, Issue 79 (3-2023)
Zanko J Med Sci 2023, 23(79): 1-10 |
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Dehghan S, Kazemipoor M, Mirhoseini M, Daneshmand F. Laboratory Comparison of the Anti-Bacterial Effect of Prosopis Farcta Extract and Hypochlorite Sodium on Enterococcus Faecalis Bacteria. Zanko J Med Sci 2023; 23 (79) :1-10
URL: http://zanko.muk.ac.ir/article-1-629-en.html
URL: http://zanko.muk.ac.ir/article-1-629-en.html
Shahid Sadoughi University of Medical Sciences
Abstract: (843 Views)
Background and Aim: The aim of root canal therapy is to eliminate intra canal irritants and prevent the reinfection of root canal. The purpose of the present study was to compare laboratory antimicrobial effect of Prosopis farcta extract with 5.25 % hypochlorite against E.faecalis.
Materials and methods: In this experimental laboratory study two solutions of 5.25% hypochlorite and seeds extract of Prosopis farcta after preparation were adjoining to oral pathogen E.faecalis. Antibacterial effect using disc diffusion and MIC method with serial dilution was assessed. The mean values of inhibition zone around discs in Mueller- Hinton agar plates were measured and compared using a ruler. Each test repeated 3 times. Data were analyzed applying SPSS (ver 17) and Kruskal-Wallis statistical test.
Results: There were significant difference in mean value of inhibition zone between Prosopis farcta extract and 5.25 % hypochlorite (P >0.0001). Bacterial growth inhibition was assessed in the MIC and MBC test, as the reported MIC was higher than 512 mg / ml because the bacteria had grown in all test tubes.
Conclusion: Considering the limitations of a laboratory study, it seems that Prosopis farcta seeds extract in comparison to 5.25 % hypochlorite has no antibacterial effect on E.faecalis.
Materials and methods: In this experimental laboratory study two solutions of 5.25% hypochlorite and seeds extract of Prosopis farcta after preparation were adjoining to oral pathogen E.faecalis. Antibacterial effect using disc diffusion and MIC method with serial dilution was assessed. The mean values of inhibition zone around discs in Mueller- Hinton agar plates were measured and compared using a ruler. Each test repeated 3 times. Data were analyzed applying SPSS (ver 17) and Kruskal-Wallis statistical test.
Results: There were significant difference in mean value of inhibition zone between Prosopis farcta extract and 5.25 % hypochlorite (P >0.0001). Bacterial growth inhibition was assessed in the MIC and MBC test, as the reported MIC was higher than 512 mg / ml because the bacteria had grown in all test tubes.
Conclusion: Considering the limitations of a laboratory study, it seems that Prosopis farcta seeds extract in comparison to 5.25 % hypochlorite has no antibacterial effect on E.faecalis.
Type of Study: Research |
Subject:
Special
Received: 2022/10/4 | Accepted: 2022/11/30 | ePublished: 2023/03/1
Received: 2022/10/4 | Accepted: 2022/11/30 | ePublished: 2023/03/1
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