Background and Aim: hospital-acquired infections have constantly been threatening for in patients. The hands with sufficient water and soap removes more than 90% of )surface contaminants(. Therefore, the aim of this study was to Microbial Pollution of liquid soap in Imam Khomeini Hospital in Divandarreh city.

Materials and Methods: In this study, 30 samples were taken from different departments of Divandarreh city hospital. The samples were transferred to laboratory with the aid of sterile swabs in sterile broth media-contained tubes. In order to incubate the samples, B.A (blood agar), EMB and Chopman media were used. After 24 hours, Samples positive in microbial contamination were separated and differentiated according to standard methods. In this differentiation, five differential media were used. Excel software was Used for data analysis.

Results: In this study, 13.3% of samples were reported positive for microbial contamination in which the largest contamination was related to Coagulase-negative staphylococci (10%). With respect to microbial contamination at different hospital departments, the delivery department showed the highest percentage of contamination.

Conclusion: According to our results, we conclude following some guidelines concerning use of hand-washing liquid and washing the hands in special respective locations, as washed the soap container after each full discharge, play a significant role in lower contamination.