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:: Volume 17, Number 52 (7-2016) ::
Zanko J Med Sci 2016, 17(52): 35-44 Back to browse issues page
Survey of tetracycline resistance genes in Shigella sonnei isolated from acute pediatric with bacterial diarrhea using Multiplex PCR method and their antibiotic resistance patterns
mrs Zahra Dolatshahi, Dr Kumarss Amini
phd Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran.
Abstract:   (403 Views)

Background and Aim: Antibiotic resistance in Shigella spp., is considered in the past two decades has increased due to indiscriminate use of antimicrobial agents. The aim of this current study was the survey of tetracycline resistance genes in Shigella sonnei isolated from acute pediatric with bacterial diarrhea using Multiplex PCR method and their antibiotic resistance patterns.

Materials and Methods: In a period of 10 months from April- January 2015, 60 Shigella spp. were isolated from non-duplicative diarrheal stool specimens. The isolates were analyzed for their antibiotic susceptibility using the CLSI guidelines M100-S14. MPCR was performed for amplification of the tetA, tetB, tetC and tetD genes. 

Results: Out of 300 tested stool samples, 60 (20%) S. sonnei were isolated. All strains (100%) were resistance to Streptomycin and Nalidixic acid. The results of antimicrobial susceptibility test showed that the percentage of resistance to tetracycline, chloramphenicol and ampicillin were 86.7%, 91.8% and 50% respectively. Eighty-five percent and 86.6% of isolates were susceptible to gentamicin and ciprofloxacin. The MPCR results revealed that 5% (3/60), 91.6% (55/60) of Shigella isolates carried tetC and tetA genes. No tetB and tetD genes were detected.

Conclusion: The tetA gene may play a role in the tetracycline resistance strain. Overall, Continuous monitoring is necessary to prevention of the spread of antibiotic resistance elements.

Keywords: tetracycline resistance genes, S. sonnei, pediatric, diarrhea antibiotic resistance.
Full-Text [PDF 947 kb]   (490 Downloads)    
Type of Study: Research | Subject: Special
Received: 2016/06/4 | Accepted: 2016/06/23 | Published: 2016/07/2
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Dolatshahi Z, Amini K. Survey of tetracycline resistance genes in Shigella sonnei isolated from acute pediatric with bacterial diarrhea using Multiplex PCR method and their antibiotic resistance patterns. Zanko J Med Sci. 2016; 17 (52) :35-44
URL: http://zanko.muk.ac.ir/article-1-113-en.html
Volume 17, Number 52 (7-2016) Back to browse issues page
مجله علوم پزشکی زانکو Zanko Journal of Medical Sciences
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