Background and Aim: Increasing resistance of bacteria to antimicrobial agents is a major problem around the world. The aim of this study was to indentify the bacterial agents in urinary tract infections and their antibiotic resistance determination in hospitalized and referred individuals to Sanandaj`s Tohid Hospital city during 2013-2014.

Materials and Methods: In this study 2406 urine samples were collected with midstream clean catch method and cultured in blood agar and Eosin-methylene blue medium using standard loop. Then incubated for 24 hsr in 37 ^oC. Identification of bacterial isolates by laboratory method and antibiotic susceptibility test were performed by disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI). For data analysis software Excel 2013 and SPSS V22 software and Fisher Exact test were used (p<0.05).

 Results: Of 2406 urine samples positive culture, 65.5% belongs to females and 34.5% belongs to males and with taking p<0.004, this difference was significant. The most common isolated pathogen was Escherichia coli and cause of 63.09% positive cultures. Most of urinary pathogens resistant were to ampicillin (64.15%), trimetoprim -sulfamethoxazole (62.67%). The least rate of resistance was to imipenem (0.7%) and amikacin (1.01%). E. coli as most common pathogen of urinary tract infections showed the most resistance to ampicillin (43.87%) and the least resistance to nitrofurantoin (3.62%) indicated.

Conclusion: When the urine culture is not available or it is not possible to wait for the antibiotic susceptibility test, antibiotics imipenem and amikacin may be the best choice to begin the treatment in the Sanandaj city.