Backgrounds and Aim: Acute menopause is important risk factors of cardiovascular disease (CVD)in women. Exercise and soy isoflavone diets have been suggested to reduce the risk of CVD in postmenopausal women. In this study, we investigated the effects the combined Resistance-Endurance training and Soybean supplementation on expression of Mir-133 in the heart ovariectomized (OVX) rat.
Materials and Methods: Fifty wistar female rats under controlled conditions (temperature, light/dark (12:12) cycle, with ad Libitum access to food and water) were housed and after familiarization with protocol they were divided into five groups, ten per group: Sham, Ovariectomy (OVX), OVX with Soy (OVX+SOY), OVX with Resistance-Endurance training (OVX+RE+EN) and OVX+RE+EN with Soy (OVX+ RE+EN+SOY). 48 hours after the end of the last session ratss were anesthetized and sacrificed. The effects of these treatments were evaluated by measuring cardiac Mir-133expression level by real time PCR method.
Results: Ovariectomy down-regulated Mir-133 gene expression; and this decrease was further in the OVX group . Six weeks Soy treatment and/or Resistance-Endurance training improved expression of Mir-133 , but the combination effect was stronger .
Conclusion: Our results suggest that the combination of Soy extract supplementation and regular Resistance-Endurance training has greater cardiac protective effect in the menopauseinduced changes than using each of them alone. Therefore, probably improved lifestyle through exercise training and the inclusion of the suitable diets such as soy isofeloven can prevent the onset of many possible disorders during menopause.