[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 15, Number 45 (9-2014) ::
Zanko J Med Sci 2014, 15(45): 9-16 Back to browse issues page
Comparison of four methods of DNA extraction from gram-negative and gram-positive bacteria
Babak Shahbazi, Hanar Narenji
Abstract:   (5224 Views)
Abstract
Background and Aim: DNA extraction is the first step in the genetic engineering research and obtains a suitable protocol for the preparation of a purified DNA which is essential. In this context, many manual and kit methods were provided. The aim of this study is to achieve a faster and low cost method for DNA extraction of Gram-positive bacteria and gram-negative bacteria. We compared four DNA extraction methods, Kit extraction, phenol chloroform, using detergent laundry brand tag and boiling. 
Materials and Methods: In this study, bacterial suspensions of Staphylococcus aureus and Vibrio cholerae were produced similar to McFarland 0.5 turbidity. Bacterial DNA was extracted using four different methods and three times for each. Using spectrophotometer and gel electrophoresis were measured concentrations and quality of DNA extraction product accordingly. In order to evaluate the efficiency of DNA extraction method, PCR, ERIC PCR and REP-PCR were performed on some of housekeeping genes downstream regions. 
Results: The concentration of the extracted DNA and results of PCR, ERIC-PCR and REP-PCR were different between gram-positive and gram-negative bacteria significantly (P< 0.05). ERIC PCR and REP-PCR efficiency of the boiling and chloroform extraction methods was disrupted only in ERIC PCR. No deficiency was observed in Sinagen kit method. 
Conclusions: The findings of this study show that despite the availability and lower cost of manual methods, these methods can be substitute for kit method.
Keywords: PCR, REP-PCR, ERIC PCR, Bacterial DNA extraction
Full-Text [PDF 504 kb]   (1924 Downloads)    
Type of Study: Research | Subject: Special
Received: 2014/07/19 | Accepted: 2014/09/3 | Published: 2014/09/16
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shahbazi B, Narenji H. Comparison of four methods of DNA extraction from gram-negative and gram-positive bacteria. Zanko J Med Sci. 2014; 15 (45) :9-16
URL: http://zanko.muk.ac.ir/article-1-32-en.html
Volume 15, Number 45 (9-2014) Back to browse issues page
مجله علوم پزشکی زانکو Zanko Journal of Medical Sciences
Persian site map - English site map - Created in 0.048 seconds with 788 queries by yektaweb 3461