Volume 16, Issue 48 (6-2015)                   Zanko J Med Sci 2015, 16(48): 42-52 | Back to browse issues page

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Golchin A, Asadpour R, Roushangar L, Kangari P. Evaluation of in vitro fertilization and early embryonic development in ghezel sheep. Zanko J Med Sci 2015; 16 (48) :42-52
URL: http://zanko.muk.ac.ir/article-1-69-en.html
Abstract:   (4922 Views)
Background and Aim: Nowadays in vitro fertilization techniques have been successfully used in the production of embryos in vitro. The embryos are then collected and used for embryo transfer, gene transfer, embryo splitting, copying, chimera, embryo freezing, sexual recognition, separation of X and Y sperm and other technologies. In vitro fertilization in ovine has mostly a research aspect. Therefore, the aim of this study was to evaluate in vitro fertilization in Ghezel sheep and to evaluate the resulting embryos for different researches. Methods: In this study, cumulus- oocyte complexes of Ghezel sheep after extraction from the ovaries of slaughtered sheep were washed three times in Tabriz abattoir. Of 338 oocytes, 201 oocytes were suitable for in vitro fertilization (IVF) and in vitro culture (IVC).The oocytes were transferred to the IVM medium and cultured in a maximum humidified, 5% CO atmosphere at 39C for 24 h. After, in vitro fertilization rate and the growth rate, they were evaluated at intervals of 48-96 hours. Results: The results of this study revealed that rate of oocyte maturation and in vitro fertilization is 95.02, 78.01 of which 46.3 % fertilized oocytes were reached to early cleavage stage. Conclusion: According to the results of this study and their comparison with other studies, fertility and embryo production rates in sheep are more than other large mammals. Therefore, Ghezel sheep embryos can be used for transgenic, chimera and embryology studies on mammals.
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Type of Study: Research | Subject: Special
Received: 2015/04/4 | Accepted: 2015/06/7 | ePublished: 2015/07/2

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